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PLANT AND ANIMAL TISSUE CULTURE

PLANT AND ANIMAL TISSUE CULTURE


Rs. 170 (सर्व कर समावेश)

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माहिती

प्रस्तावना

Plant Tissue Culture
1. Introductory History
1.1 Concepts of Cell Theory
1.2 Cellular Totipotency
1.3 Milestones in Plant Tissue Culture
1.4 The General Technique
1.4.1 Important Features of Tissue Culture Technique
1.4.2 Importance of Tissue Culture Technique
1.5 Applications of Plant Tissue Culture
2. Infrastructure and Organization of Plant Tissue Laboratory
2.1 General Requirements of Tissue Culture Laboratory
2.2 Equipments and Instruments used in Plant Tissue Culture
2.3 Different Work Areas
2.3.1 Culture Room
2.3.2 Bottle Wash Machine
2.3.3 Hand and Leg Wash Room
2.3.4 Dressing Room
2.3.5 Culture Transfer Room
3. Aseptic Techniques
3.1 Introduction
3.2 Washing and Preparation of Glass Wares
3.3 Sterilization
3.4 Precautions to Maintain Aseptic Conditions
4. Culture Medium
4.1 Nutritional Requirements of the Explants
4.1.1 Composition of M. S. Medium
4.1.2 Plant Growth Regulators (PGR’s) and their In Vitro Roles
4.2 Preparation of Medium
4.3 Importance of Mineral Components in the Nutrient Medium
5. ‘Explant’ For Plant Tissue Culture
5.1 Explant
5.2 Plant Histology
5.3 Response of Explants In Vitro - Dedifferentiation & Redifferentiation
6. Callus Culture Technique
6.1 Introduction
6.2 Principle
6.3 Callus Cultures
6.3.1 Protocol for the Culture of Plant Tissues
6.3.2 Significance of Callus Culture
6.3.3 Factors affecting Callus Culture
6.3.4 Internal Structure of Callus
7. Suspension Culture Technique
7.1 Introduction
7.2 Protocol for the Establishment of Suspension Culture
7.3 Types of Suspension Cultures
7.4 Measurement of Growth
7.4.1 Cell Packing Method
7.4.2 Direct Cell Count Method
7.4.3 Direct Weight Method
7.4.4 Protein Measurement Technique
7.5 Experiments to Assess the Cell Viability
7.5.1 Evan’s Blue Dye treatment
7.5.2 Chemical Method
7.6 Synchronization
7.7 Importance and Uses of Suspension Culture
8. Organ Culture Technique
8.1 Shoot Tip Culture
8.2 Root Tip Cultures
8.3 Leaf Culture
8.4 Meristem Culture
9. Anther and Pollen Culture Technique
9.1 Anther Culture
9.2 Pollen Culture
9.2.1 Protocol for Pollen Culture Technique
9.2.2 Advantages of Pollen Culture over Anther Culture
9.2.3 Pollen Biotechnology
10. Ovary, Ovule, Embryo and Endosperm Culture
10.1 Ovary Culture
10.2 Ovule Culture
10.3 Embryo Culture
10.4 Endosperm
11. Protoplast
11.1 Methods of Isolation of Protoplasts
11.2 Maintenance of Protoplasts
11.3 Culture of Protoplasts
11.3.1 Viability Tests for Protoplasts
11.3.2 Regeneration of Cell Wall
11.3.3 Cell Division and Callus Formation
11.3.4 Importance of Protoplast Culture
11.4 Somatic Hybridization
11.4.1 Protoplast Fusion
11.4.2 Selection of Hybrid Protoplasts
11.4.3 Regeneration of Plantlets
11.4.4 Production of Symmetric and Asymmetric Hybrids
11.4.5 Cybrids
11.5 Genetic Transformations
11.5.1 Gene Manipulation in Plants
11.5.2 Direct DNA Transfer Methods
11.5.3 Transformation of Protoplasts
11.5.4 Agrobacterium Mediated Gene Transfer
11.5.5 Integration and Expression of Foreign DNA
11.5.6 Applications in Crop Improvement
12. Different Routes of Multiplication In Vitro
12.1 Micropropagation (In Vitro Multiplication)
12.2 Methods of Micropropagation
12.2.1 Proliferation of Auxiliary Buds
12.2.2 Induction of Adventitious Buds, Bulbs and Protocorms
12.2.3 Somatic Embryogenesis
12.2.4 Meristem Culture
12.3 Applications
12.4 Limitation
12.5 Production of Artificial Seeds
12.5.1 Techniques to Produce Artificial Seeds
12.5.2 Types of Artificial Seeds
12.5.3 Salient Features of Artificial Seeds
13. Somaclonal Variation
13.1 Introduction
13.1.1 Terminology
13.1.2 Origin of Somaclonal Variation
13.2 Isolation of Somaclonal Variants
13.2.1 Advantages
13.2.2 Limitations
13.3 Germplasm Preservation
13.4 Mechanisms Underlying Genetic Variation
14. Introduction To Secondary Metabolite production In Vitro Biotransformations
14.1 Introduction
14.2 Culture of Plant Cells for the Extraction of Secondary Metabolism
14.3 Commercial Aspects
14.4 Production of Callus for the Extraction of Secondary Metabolites
14.5 Culture Adjustments for the Extraction of Specific Substances
14.6 Biotransformation in Plants
14.6.1 Elicitor - Dependent Biosynthesis
14.6.2 Immobilization of Plant Cells
14.7 Optimisation of the Yield
14.8 Applications
14.9 Advantages and Limitations
Animal tissue culture
1. Animal Tissue Culture
1.1 Introduction
1.2 Basic Requirement for Cell Culture Laboratory
1.3 Source of Explant Tissues
1.4 Collection of Explants
1.5 Precautions
1.6 Maintainance of Aseptic Condition in Cell Culture Laboratory
2. NUTRITION and Physiology Media Components
2.1 Introduction
2.1.1 Natural Media
2.1.2 Artificial Media
2.2 Preparation and Sterilization of Substrate and Medium
3. Primary Cell Culture
3.1 Introduction
3.1.1 Sterilization of Explant Tissues
3.1.2 Culture of Explants
3.1.3 Dissociation Method
3.2 lymphocytes
4. Cell Lines
4.1 Introduction
4.2 Immortalization of Cell Lines
4.3 Cell Line Designations
4.4 Selection of Cell Line
4.5 Routine Maintenance
4.6 Replacement of Medium
4.7 Animal Cell Subculture
4.7.1 Criteria for Subculture
4.7.2 Examples of Cell Lines
4.8 Insect Cell Culture
4.8.1 Applications
4.8.2 Method
5. Organ and Tissue Culture
5.1 Organ Culture
5.1.1 Technique
5.1.2 Advantages
5.1.3 Limitations
5.1.4 Applications
5.2 Tissue Culture
6. Karyotyping
6.1 Definition
6.2 Chromosomal Analysis (Karyotyping) from the appropriate Sample
6.2.1 Human Karyotype
6.2.2 Characterization
6.3 Chromosome Staining
6.4 Banding Technique
6.4.1 Conventional Staining
6.4.2 G Bands by Trypsin using Giemsa (GTG) Banding
6.4.3 C Bands using Barium Hydroxide Giemsa (CBG) Banding
6.4.4 Silver Staining of Nucleolar Organiser Regions (AgNOR)
6.4.5 Q Bands by Fluorescence using Quinacrine (QFQ)
6.5 Applications of Karyotyping
6.6 Some Examples of Karyotypes
6.6.1 Klinefelter Syndrome
6.6.2 Turner’s Syndrome
7. Cell Repositories and their Function
7.1 Introduction
7.2 Functions of Cell Banks
7.3 Maintenance of Cell Lines
7.4 Precautions during Cryopreservation
8. Application of Animal Cell Culture
8.1 Vaccines
8.2 Interferons
8.3 Recombinant Proteins
8.4 Hybrid Antibodies
8.5 Antibodies
8.5.1 Production of Monoclonal Antibodies
8.5.2 Applications of Monoclonal Antibodies

शैक्षणिक माहिती

  • शैक्षणिक टप्पा पदवीधर

  • विद्याशाखा विज्ञान

  • शाखा Biotechnology

  • इयत्ता दुसरे वर्ष

  • सत्र Ii

  • माध्यम इंग्रजी

  • मंडळ/विद्यापीठ पुणे

  • विषय Plant And Animal Tisuue Culture

अभिप्राय

  • या वस्तूबद्दल शेरा/अभिप्राय उपलब्ध नाही. अभिप्राय लिहिणारे सर्वप्रथम व्हा.
शिपिंग बद्दल माहिती
  • लागू असलेल्या ऑर्डरवर फ्री शिपिंगचा आनंद घ्या:
    • पुण्यामध्ये 3०० पेक्षा अधिक किमतीच्या खरेदीवर
    • महाराष्ट्रात 500 पेक्षा अधिक किमतीच्या खरेदीवर
    • भारतात 1000 पेक्षा जास्त किमतीच्या खरेदीवर
  • सामान्यतः 4-5 व्यावसायिक दिवसात डिलेव्हरी होते
  • कॅश ऑन डिलिव्हरी सेवा उपलब्ध
आपल्यासाठी विशेष ऑफर
  1. ऑनलाइन ऑर्डर्सवर विशेष ऑफर
  2. पुस्तके आणि स्टेशनरीवर उत्कृष्ट सवलत मिळवा

PLANT AND ANIMAL TISSUE CULTURE

PLANT AND ANIMAL TISSUE CULTURE